产品详情
英文名称 Phospho-SATB1 (Ser47)
中文名称 磷酸化核基质结合区结合蛋白1抗体
别 名 DNA binding protein SATB1; DNA-binding protein SATB1; SATB homeobox 1; SATB1; SATB1_HUMAN; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA); Special AT rich sequence binding protein 1; Special AT-rich sequence-binding protein 1.
磷酸化核基质结合区结合蛋白1抗体
说 明 书 0.1ml
产品类型 磷酸化抗体
研究领域 免疫学 染色质和核信号 转录调节因子 结合蛋白 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Chicken, Dog, Pig, Cow, Rabbit,
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 86kDa
细胞定位 细胞核
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human SATB1 around the phosphorylation site of Ser47
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 磷酸化核基质结合区结合蛋白1抗体产品介绍 background:
SATB1 selectively binds double-stranded, special AT-rich RNA sequences in which 1 strand exclusively consists of well-mixed A, T, and C nucleotides (ATC sequences). It complexes mainly with p300 and this complex can bind to the gp91phox promoter and repress transcriptional activity. Its ability to repress transcription is regulated by nuclear matrix binding. It is expressed predominantly in the thymus.
Function:
Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
Subunit:
Interacts (via DNA-binding domains) with CUX1; leading to inhibit the attachment to DNA (By similarity). Homodimer. Part of the nuclear protein complex gamma-globin promoter and enhancer binding factor (gamma-PE) composed at least by SATB1 and HOXB2. Interaction with CtBP1 when not acetylated stabalizes attachment to DNA and promotes transcription repression. Interacts with PCAF. Interacts with sumoylated PML, HDAC1 and HIV-1 Tat via the PDZ-like dimerization domain. Interacts also with DYNLT3 and POLR2J2. Binds to EP300.
Subcellular Location:
Nucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies (PML NBs).
Tissue Specificity:
Expressed predominantly in thymus.
Post-translational modifications:
Sumoylated. Sumoylation promotes cleavage by caspases.
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
Similarity:
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
Gene ID:
6304
Database links:
Entrez Gene: 6304 Human
Entrez Gene: 20230 Mouse
Entrez Gene: 316164 Rat
Omim: 602075 Human
SwissProt: Q01826 Human
SwissProt: Q60611 Mouse
Unigene: 517717 Human
Unigene: 311655 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化核基质结合区结合蛋白1抗体
hz-2163R RNF148 环指蛋白148抗体
hz-6096R RNF139 环指蛋白139抗体
hz-4104R FXYD1 磷酸神经膜抗体
hz-1166R ROCK1 Rho相关蛋白激酶1抗体
hz-4630R phospho-ROCK1(Thr455/Ser456) 磷酸化Rho相关蛋白激酶1抗体
hz-1205R ROCK2 Rho相关蛋白激酶2抗体
hz-5674R phospho-ROCK2(Thr396) 磷酸化Rho相关蛋白激酶2抗体
hz-5675R phospho-ROCK2(Thr249) 磷酸化Rho相关蛋白激酶2抗体
hz-5676R phospho-ROCK2(Tyr256) 磷酸化Rho相关蛋白激酶2抗体
hz-1886R RPLP0 酸性核糖体磷蛋白大亚基P0抗体
hz-2504R ROS/c ros 活性氧簇ROS抗体
hz-3863R ROR Gamma/RORC/NR1F3 孤儿核受体抗体
hz-3565R RPS6 S6核糖体蛋白抗体
hz-3388R Phospho-S6 Ribosomal Protein (Ser235+Ser236) 磷酸化S6核糖体蛋白抗体
hz-3389R Phospho-S6 Ribosomal Protein (Ser240/244) 磷酸化S6核糖体蛋白抗体
hz-2777R Rb/P105 RB 成视网膜细胞瘤基因抗体
hz-1347R phospho-Rb/p105-Rb(Ser780) 磷酸化成视网膜细胞瘤抑癌蛋白抗体
hz-5348R phospho-FXYD1 (Ser83) 磷酸化磷酸神经膜抗体
hz-0278R RRAD RRAD抗体
hz-5349R phospho-FXYD1 (Ser88) 磷酸化磷酸神经膜抗体
hz-3554R Rsk2/MAPKAP Kinase 1b 核糖体S6激酶RSK2抗体
hz-3367R Phospho-RSK2 (Ser227) 磷酸化核糖体S6激酶RSK2抗体
hz-3368R Phospho-RSK2(Tyr529) 磷酸化核糖体S6激酶RSK2抗体
hz-3553R RSK3/Ribosomal S6 kinase 3 核糖体蛋白S6激酶家族RSK3抗体
hz-3365R Phospho-RSK3 (Thr353/356) 磷酸化核糖体蛋白S6激酶家族RSK3抗体
hz-3366R Phospho-RSK3 (Thr356/Ser360) 磷酸化核糖体蛋白S6激酶家族RSK3抗体
hz-1264R RSV 呼吸道合胞病毒抗体
hz-1379R RXR Alpha/RXRA/Retinoid X receptor 核受体RXRα抗体
hz-2802R RXR gamma 维甲酸受体G抗体
hz-6219R RXR beta/RXRB 核受体RXRβ抗体
hz-2744R RUNX1/AML1 急性髓细胞白血病1蛋白抗体
hz-3024R phospho-RUNX1(Ser266) 磷酸化急性髓细胞白血病1蛋白抗体
hz-0378R Runx3 Runx3抗体
hz-2018R SDH/S6PDH 6-磷酸山梨醇脱氢酶抗体