产品详情
英文名称 phospho-RAC1 + RAC2 (Ser71)
中文名称 磷酸化细胞迁移诱导因子5抗体
别 名 RAC1(phospho S71); RAC2(phospho S71); RAC1 + RAC2 (phospho Ser71); Cell migration inducing gene 5 protein; Cell migration-inducing gene 5 protein; MIG5; MIG 5; Migration inducing gene 5; Migration inducing protein 5; p21 Rac1; Rac 1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1; Rho family small GTP binding protein Rac1; TC 25; TC25; MGC111543; p21-Rac1; RAC1; RAC1_HUMAN; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; rho family small GTP binding protein Rac1.
磷酸化细胞迁移诱导因子5抗体
说 明 书 0.1ml
产品类型 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 G蛋白偶联受体
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 22kDa
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RAC1 around the phosphorylation site of Ser71
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
磷酸化细胞迁移诱导因子5抗体产品介绍 background:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function:
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Subunit:
Interacts with NISCH. Interacts with PIP5K1A. Interacts with the GTP-bound form of RAB7A. Interacts with SRGAP2. Interacts with CYFIP1/SRA-1. Interacts with PLXNB3. Interacts with ARHGDIA; the interaction is induced by SEMA5A, mediated through PLXNB3 and inactivates and stabilizes RAC1. Interacts (GTP-bound form preferentially) with PKN2 (via the REM repeats); the interaction stimulates autophosphorylation and phosphorylation of PKN2. Interacts with the GEF proteins PREX1, RASGRF2, FARP1, FARP2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM; the interaction requires PAK1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with TBC1D2. Interacts with UNKL. Interacts with USP6. Interacts with SPATA13. Interacts with ARHGEF16; mediates activation of RAC1 by EPHA2. Interacts with ITGB4. Interacts with S100A8 and calprotectin (S100A8/9). Interacts with PACSIN2. Interacts with ITGB1BP1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane.
Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts.
Tissue Specificity:
Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Post-translational modifications:
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome.
Similarity:
Belongs to the small GTPase superfamily. Rho family.
Gene ID:
5879
Database links:
Entrez Gene: 5879 Human
Entrez Gene: 5880 Human
Entrez Gene: 19353 Mouse
Entrez Gene: 19354 Mouse
Entrez Gene: 363875 Rat
Entrez Gene: 366957 Rat
Omim: 602048 Human
Omim: 602049 Human
SwissProt: P15153 Human
SwissProt: P63000 Human
SwissProt: P63001 Mouse
SwissProt: Q05144 Mouse
SwissProt: Q6RUV5 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化细胞迁移诱导因子5抗体
hz-3140R Phospho-FoxO3a (Ser253) 磷酸化叉头蛋白3A抗体
hz-2766R FOXO4 叉头蛋白O4抗体
hz-3143R Phospho-FoxO4 (Ser193) 磷酸化叉头蛋白4抗体
hz-3144R phospho-FOXO4 (Ser197) 磷酸化叉头蛋白4抗体
hz-3145R phospho-FOXO4 (Ser262) 磷酸化叉头蛋白4抗体
hz-3654R FPRL1/RFP/Lipoxin A4 receptor 脂氧素受体1抗体
hz-0886R Measles virus fusion protein 麻疹病毒融合蛋白抗体
hz-5952R POMT1 蛋白甘露糖基转移酶1抗体
hz-5953R RALA Ras样蛋白A抗体
hz-0507R TBX2/T-box2 血栓素B2抗体(一种新发现的抑癌基因)
hz-0895R FSH receptor 促卵泡刺激素受体抗体
hz-1773R Follistatin 卵泡抑素抗体
hz-1770R FSP/Spastin 纤维母细胞表面蛋白抗体
hz-1536R FSH/FSHB 促卵泡刺激素抗体
hz-1769R FHIT 脆性组氨酸三联体抗体
hz-3146R Phospho-FHIT (Tyr114) 磷酸化脆性组氨酸三联体抗体
hz-4044R Glucose 6 phosphatase alpha 葡萄糖6磷酸酶α抗体
hz-3987R G6PD/Glucose 6 Phosphate Dhzydrogenase 葡萄糖-6磷酸脱氢酶抗体
hz-0348R G protein beta subunit GI G蛋白/鸟苷酸结合蛋白抗体
hz-5954R RASA3 Ras-GTP酶激活蛋白3抗体
hz-3941R G protein beta 1/GNB1 G蛋白β1抗体/鸟嘌呤核苷酸结合蛋白β亚基1
hz-3942R G protein beta 2/GNB2 G蛋白β2抗体/鸟嘌呤核苷酸结合蛋白β亚基2
hz-3943R G protein beta 3/GNB3 G蛋白β3抗体/鸟嘌呤核苷酸结合蛋白β亚基3
hz-3940R G protein beta 4/GNB4 G蛋白β4抗体/鸟嘌呤核苷酸结合蛋白β亚基4
hz-3519R SLK/Fyn 酪氨酸蛋白激酶Fyn(同步原癌基因)抗体
hz-5332R phospho-SLK/FYN (Tyr530) 磷酸化酪氨酸蛋白激酶Fyn/同步原癌基因抗体
hz-2252R GABA 兔抗γ1氨基丁酸抗体
hz-3766R GARB1/GABAR γ1氨基丁酸受体β1抗体
hz-5140R GABPB2 核呼吸因子2抗体
hz-2770R Gab1 接头蛋白Gab 1抗体
hz-3165R Phospho-Gab1 (Tyr627) 磷酸化接头蛋白Gab 1抗体
hz-3166R Phospho-Gab1 (Tyr659) 磷酸化接头蛋白Gab 1抗体
hz-2885R Gab2 接头蛋白Gab 2抗体
hz-3177R phospho-GAB2(Ser623) 磷酸化接头蛋白Gab2抗体
hz-3178R phospho-GAB2 (Tyr452) 磷酸化接头蛋白Gab 2抗体
hz-3182R phospho-GAB2 (Tyr643) 磷酸化接头蛋白Gab2抗体