产品详情
英文名称 RCC1
中文名称 染色体浓缩调控蛋白1抗体
别 名 Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN.
染色体浓缩调控蛋白1抗体
说 明 书 0.2ml
研究领域 细胞生物 染色质和核信号 信号转导 转录调节因子 激酶和磷酸酶
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 46kDa
细胞定位 细胞核 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human RCC1 (2-50aa)
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
染色体浓缩调控蛋白1抗体 产品介绍 background:
Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.
Function:
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Subunit:
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
Subcellular Location:
Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis.
Post-translational modifications:
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Similarity:
Contains 7 RCC1 repeats.
Gene ID:
1104
Database links:
Entrez Gene: 1104 Human
Entrez Gene: 100088 Mouse
Entrez Gene: 682908 Rat
Omim: 179710 Human
SwissProt: P18754 Human
SwissProt: Q8VE37 Mouse
Unigene: 469723 Human
Unigene: 255045 Mouse
Unigene: 217123 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
染色体浓缩调控蛋白1抗体
hz-1001R E2F2 转录因子E2F-2抗体
hz-5857R ADAMTS15 整合素样金属蛋白酶与凝血酶15型抗体
hz-1722R E2F3 转录因子E2F-3抗体
hz-1399R E2F4 转录因子E2F-4抗体
hz-1734R E2F5 转录因子E2F-5抗体
hz-1750R E2F6 转录因子E2F6抗体
hz-2305R E2EPF 泛素交联酶抗体
hz-4265R E2F8 转录因子E2F8抗体
hz-2071R E2 tag E2 tag标签抗体
hz-0714R HPV16 E7 protein 人乳头瘤病毒16型E7抗体
hz-5860R ADAMTS9 整合素样金属蛋白酶与凝血酶9型抗体
hz-5861R ADAMTSL1 整合素样金属蛋白酶与凝血酶样1蛋白抗体
hz-1003R EAAT1/Glast 胶质细胞谷氨酸运载蛋白1抗体
hz-1751R EAAT2 胶质细胞谷氨酸运载蛋白2抗体
hz-1752R EAAT3 胶质细胞谷氨酸运载蛋白3抗体(神经/上皮细胞谷氨酸运载蛋白)
hz-0820R EBNA 3A EB病毒核抗原-3A抗体
hz-5863R ADAMTSL3 整合素样金属蛋白酶与凝血酶样2蛋白抗体
hz-1190R ECE1 内皮素转化酶1抗体
hz-1753R ECE2 内皮素转化酶2抗体
hz-0500R ECG2 食道癌相关基因抗体
hz-5872R KLKB1/Plasma Kallikrein 1B 血浆激肽释放酶抗体
hz-5058R ECHS1 烯脂酰辅酶A水解酶抗体
hz-4058R hzHADH 三羟酰辅酶A脱氢酶抗体
hz-0776R ECM1 细胞外基质蛋白1抗体
hz-5864R ADAMTSL4 整合素样金属蛋白酶与凝血酶样4蛋白抗体
hz-1754R ECP 嗜酸性粒细胞阳离子蛋白抗体
hz-4102R ECT2 上皮细胞癌转化蛋白2抗体
hz-5344R phospho-ECT2 (Thr790) 磷酸化上皮细胞癌转化蛋白2抗体
hz-1149R ED-1 外胚层发育不良抗体
hz-5873R CNDP1 肌肽二肽酶1抗体
hz-1757R EDNRA 内皮素受体A抗体
hz-2363R EDNRB 内皮素受体B抗体
hz-3611R EEF2 真核翻译延长因子2抗体
hz-3486R Phospho-EEF2 (Thr56) 磷酸化真核翻译延长因子2抗体
hz-3612R EEF2k 真核延伸因子激酶2抗体
hz-3487R Phospho-EEF2k (Ser366) 磷酸化真核延伸因子激酶2抗体
hz-4651R Alpha B Crystallin/HSPB5 热休克蛋白β5抗体