泛素交叉反应蛋白(UCRP)重组蛋白

物种Homo sapiens (Human，人)

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量20.7kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Gly2~Gly157 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 90%

泛素交叉反应蛋白(UCRP)重组蛋白等电点-

应用SDS-PAGE; WB; ELISA; IP.

Recombinant Ubiquitin Cross Reactive Protein (UCRP)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Gly2~Gly157

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P05161

Host: E. coli

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

泛素交叉反应蛋白(UCRP)重组蛋白(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.0

Predicted Molecular Mass: 20.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

泛素交叉反应蛋白(UCRP)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

GWDLTVKML AGNEFQVSLS SSMSVSELKA QITQKIGVHA FQQRLAVHPS GVALQDRVPL

ASQGLGPGST VLLVVDKCDE PLSILVRNNK GRSSTYEVRL TQTVAHLKQQ VSGLEGVQDD

LFWLTFEGKP LEDQLPLGEY GLKPLSTVFM NLRLRGG