参考价: 面议
  • 品牌:
  • 规格:
  • 供货周期:
logo
上海康朗生物科技有限公司
进入展位

产品详情

含RNA结合冷休克域蛋白E1(CSDE1)重组蛋白


来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量31.0kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Met1~Val245 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)

性状冻干粉

纯度> 95%

等电点-

含RNA结合冷休克域蛋白E1(CSDE1)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Recombinant Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Met1~Val245

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O75534

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 31.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

含RNA结合冷休克域蛋白E1(CSDE1)重组蛋白[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

含RNA结合冷休克域蛋白E1(CSDE1)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

MSFDPNLLHN NGHNGYPNGT SAALRETGVI EKLLTSYGFI QCSERQARLF FHCSQYNGNL

QDLKVGDDVE FEVSSDRRTG KPIAVKLVKI KQEILPEERM NGQVVCAVPH NLESKSPAAP

GQSPTGSVCY ERNGEVFYLT YTPEDVEGNV QLETGDKINF VIDNNKHTGA VSARNIMLLK

KKQARCQGVV CAMKEAFGFI ERGDVVKEIF FHYSEFKGDL ETLQPGDDVE FTIKDRNGKE

VATDV