血管生长素(ANG)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位分泌

预测分子量15.9kDa

实际分子量18kDa(差异分析请参阅说明书)

片段与标签Ala24~His148 with N-terminal His Tag

缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

性状冻干粉

纯度> 97%

血管生长素(ANG)重组蛋白Recombinant Angiogenin (ANG)

Organism Species: Bos taurus; Bovine (Cattle)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ala24~His148

Tags: N-terminal His-Tag

Tissue Specificity: Liver. Subcellular Location: Secreted. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.2

Predicted Molecular Mass: 15.9kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex.

血管生长素(ANG)重组蛋白[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

血管生长素(ANG)重组蛋白[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE

等电点9.2