产品详情
层粘连蛋白α1(LAMα1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量17.8kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Ala886~Val1039 (Accession # P25391) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
层粘连蛋白α1(LAMα1)重组蛋白应用SDS-PAGE; WB; ELISA;
Laminin Alpha 1 (LAMa1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Ala886~Val1039 (Accession # P25391),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 17.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
层粘连蛋白α1(LAMα1)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-ADGFY GDAVTAKNCR ACECHVKGSH SAVCHLETGL CDCKPNVTGQ
QCDQCLHGYY GLDSGHGCRP CNCSVAGSVS DGCTDEGQCH CVPGVAGKRC
DRCA HG FYAY QDGS C TP CD C PHTQN T CDPE TGE CV CPP HT QGVK CEE CE D
GHWGYDAEV
层粘连蛋白α1(LAMα1)重组蛋白[ REFERENCES ]
1. Chen R., et al. (2009) J. Proteome Res. 8:651-661.
2. Nissinen M., et al. (1991) Biochem. J. 276:369-379.
3. Haaparanta T., et al. (1991) Matrix 11:151-160.
4. Olsen D., et al. (1989) Lab. Invest. 60:772-782.
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