二氢硫辛酸转乙酰基酶(DLAT)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位线粒体

预测分子量29.0kDa

实际分子量29kDa(差异分析请参阅说明书)

片段与标签Thr368~Trp626 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 97%

等电点8.4

二氢硫辛酸转乙酰基酶(DLAT)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.

Recombinant Dihydrolipoyl Transacetylase (DLAT)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Thr368~Trp626

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Heart, Kidney, Liver. Subcellular Location: Mitochondrion matrix. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 5% trehalose and 0.01% sarcosyl. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.4

Predicted Molecular Mass: 29.0kDa

Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

二氢硫辛酸转乙酰基酶(DLAT)重组蛋白[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

二氢硫辛酸转乙酰基酶(DLAT)重组蛋白[ IDENTIFICATION ]

Figure 1. SDS-PAGE