谷氨酸脱羧酶2(GAD2)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic sid

预测分子量36.1kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Arg65~Arg105 (Accession # Q05329) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点6.4

谷氨酸脱羧酶2(GAD2)重组蛋白应用SDS-PAGE; WB; ELISA; IP.

Recombinant Glutamate Decarboxylase 2, Acid (GAD2)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Arg65~Arg105 (Accession # Q05329),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Cytoplasm, cytosol.

Cytoplasmic vesicle. Cell junction, synapse,

presynaptic cell membrane; Lipid-anchor. Golgi

apparatus membrane; Peripheral membrane

protein; Cytoplasmic side. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 36.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

谷氨酸脱羧酶2(GAD2)重组蛋白[ USAGE ]

Reconstitute in ddH2O. [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

谷氨酸脱羧酶2(GAD2)重组蛋白[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its

sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- RAAARK AACACDQKPC

SCSKVDVNYA FLHATDLLPA CDGER

[ REFERENCES ]

1. Karlsen A.E., et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88:8337-8341. 2. Bu D.-F., et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:2115-2119. 3. Bu D.-F., Tobin A.J. (1994) Genomics 21:222-228. 4. Kim J., et al. (1993) Diabetes 42:1799-1808.