神经氨酸酶(NEU)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量21.6kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Ala141~His286 (Accession # Q99519) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 90%

等电点-

神经氨酸酶(NEU)重组蛋白应用SDS-PAGE; WB; ELISA; IP.

Recombinant Neuraminidase (NEU)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Ala141~His286

Tags: Two N-terminal Tags, His-tag and S-tag

Accession: Q99519

Host: E. coli

Subcellular Location: Lysosome membrane;

Peripheral membrane protein; Lumenal side.

Lysosome lumen. Cell membrane. Cytoplasmic

vesicle.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM

PBS, pH7.4, containing 1mM DTT, 5% trehalose,

0.01% sarcosyl and preservative.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 21.6kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

神经氨酸酶(NEU)重组蛋白[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

神经氨酸酶(NEU)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

AVVSDVETGV VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSTPRNLSLD IGTEVFAPGP

GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGASW RYGSGVSGIP

YGQPKQENDF NPDECQPYEL PDGSVVINAR NQNNYH

[ REFERENCES ]

1. Chen Y., et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:542-553.

2. Offner G.D., et al. (1998) Biochem. Biophys. Res. Commun. 251:350-355.

3. Desseyn J.-L., et al. (1998) J. Biol. Chem. 273:30157-30164.

4. Desseyn J.-L., et al. (1997) J. Biol. Chem. 272:3168-3178.