转换蛋白2(TNP2)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量16.8kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Met1~Lys117 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点-

转换蛋白2(TNP2)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Recombinant Transition Protein 2 (TNP2)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Met1~Lys117

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P11378

Host: E. coli

Subcellular Location: Nucleus. Chromosome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 11.8

Predicted Molecular Mass: 16.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

转换蛋白2(TNP2)重组蛋白[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

转换蛋白2(TNP2)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

MDTKMQS LPT THP HP HSSS R PQS HTS NQCN QCT CS HHCRS CSQAGHAGSS

SS PS P GPPMK HP K PS V HS R H SPA RPS H RGS CP K NRK T FE G KV SK RKAV R R

RKRTHRAKRR TSGRRYK