Quantity size:

0.2ml

Concentration:

1mg/ml  扑克蒙蛋白抗体 Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic扑克蒙蛋白抗体 mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

扑克蒙蛋白抗体Also known as:

ZBTB7; ZBTB7A; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI1; HIV-1 1st-binding protein 1; Leukemia/lymphoma related factor; LRF; Pokemon; TIP21; TTF-I interacting peptide 21; Zinc finger and BTB domain-containing protein 7A; ZBT7A_HUMAN.

Specificity:

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Rabbit Polyclonal IgG,扑克蒙蛋白抗体 affinity purified by Protein A.

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Reacts with: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, .

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Immunogen: KLH conjugated synthetic peptide derived from human Pokemon.

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Predicted Molecular Weight: 63kDa.

扑克蒙蛋白抗体Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user

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kl302Hu01N-酰基鞘氨醇酰胺水解酶1(ASAH1)多克隆抗体Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)

kl303Mu01转录激活因子3(ATF3)多克隆抗体Polyclonal Antibody to Activating Transcription Factor 3 (ATF3)

kl304Hu01吸引素(ATRN)多克隆抗体Polyclonal Antibody to Attractin (ATRN)

kl310Hu01腺嘌呤磷酸核糖转移酶(APRT)多克隆抗体Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT)

kl314Hu01腺瘤息肉病大肠杆菌蛋白(APC)多克隆抗体Polyclonal Antibody to Adenomatosis Polyposis Coli Protein (APC)

kl317Hu01精氨基琥珀酸合成酶1(ASS1)多克隆抗体Polyclonal Antibody to Argininosuccinate Synthetase 1 (ASS1)

kl319Hu01芳香酶(ARO)多克隆抗体Polyclonal Antibody to Aromatase (ARO)

kl319Ra01芳香酶(ARO)多克隆抗体Polyclonal Antibody to Aromatase (ARO)

kl321Hu01无孢蛋白(ASPN)多克隆抗体Polyclonal Antibody to Asporin (ASPN)

kl321Mu01无孢蛋白(ASPN)多克隆抗体Polyclonal Antibody to Asporin (ASPN)

kl322Hu01自身免疫调节因子(AIRE)多克隆抗体Polyclonal Antibody to Autoimmune Regulator (AIRE)

kl322Mu01自身免疫调节因子(AIRE)多克隆抗体Polyclonal Antibody to Autoimmune Regulator (AIRE)

kl323Hu01外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)多克隆抗体Polyclonal Antibody to Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)

kl324Hu01Bcl2拮抗/杀伤因子1(BAK1)多克隆抗体Polyclonal Antibody to Bcl2 Antagonist/Killer 1 (BAK1)

kl332Hu01核有丝分裂器蛋白1(NUMA1)多克隆抗体Polyclonal Antibody to Nuclear Mitotic Apklatus Protein 1 (NUMA1)

kl335Hu01Bcl2关联永生基因3(BAG3)多克隆抗体Polyclonal Antibody to Bcl2 Associated Athanogene 3 (BAG3)

kl340Hu01胱天蛋白酶6(CASP6)多克隆抗体Polyclonal Antibody to Caskle 6 (CASP6)

kl342Hu01胆盐依赖性脂肪酶(BSDL)多克隆抗体Polyclonal Antibody to Likle, Bile Salt Dependent (BSDL)

kl343Hu0170kDa热休克蛋白5(HSPA5)多克隆抗体Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

kl343Mu0170kDa热休克蛋白5(HSPA5)多克隆抗体Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)