产品详情
SuperCos I噬菌体展示质粒
基本信息
启动子: SV40,T7,T3
复制子: pUC ori
质粒分类: 哺乳细胞,蛋白过表达载体
质粒大小: 7,9kb
原核抗性: Amp
筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: T7:TAATACGACTCACTATAGGG
3'测序引物: BGH:TAGAAGGCACAGTCGAGG
质粒简介
SuperCos 1 is a novel, 7.9-kb cosmid vector that contains bacteriophage promoter sequences flanking a unique cloning site (see Figure 1). This structure allows rapid synthesis of "walking" probes specific for the extreme ends of insert DNA. The SuperCos 1 vector is also engineered to contain genes for the amplification and expression of cosmid clones in eukaryotic cells. In addition, most genomic inserts can be excised as a single large restriction fragment using the Not I restriction site that flanks the SuperCos 1 polylinker.
质粒图谱
SuperCos I噬菌体展示质粒使用说明:
1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min;
2、从-80℃冰箱中取出相应的感受态,置于冰盒上解冻,并做好标记;
3、取2μl质粒加至100μl感受态中,冰浴30min;
4、42℃热激90s,再冰浴2min;
5、加入900μl无抗的LB液体培养基,180rpm震荡培养45min;
6、6000rpm离心5min,仅留100ul上清混匀菌体沉淀;
7、混匀后的菌液加至对应抗性的LB平板上,倒入适量玻璃珠,涂匀液体;
8、将平板正向培养1h,再倒置培养12h~16h;
9、挑取单克隆菌落至对应抗性的LB液体培养基中,震荡培养12h~16h,根据实验需要提取质粒。
SuperCos I噬菌体展示质粒注意事项:
1、如果您收到的是甘油菌种,请先四区划线,挑取单克隆培养。
2、如果第二天转化平板长的过多,请将质粒按比例稀释后再转化。
问商家