产品详情
产品信息
货号 | 名称 | 产品形式 | 规格 | 储存 |
QC1558 | S17-1λpir | 5ug | -20℃ |
使用说明
收到甘油菌后,请及时在固体平板上划线培养,挑取单菌落接种到液体培养基中,震荡培养后使用并保存甘油菌。
基本信息
抗性:Kan
菌株类别:大肠杆菌
培养条件:37℃,有氧,LB
质粒转化:42℃热激
保存方式:20%甘油,-80℃
基本应用:用于基因克隆
备注:S17-1 lamp pir是一种含pir基因的结合转移菌株
菌株简介
E. coli S17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary).E. coli S17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary). The genotype of the strain is TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir.
注意事项
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅可用于实验室研究,不能用于动物,人体以及作为食品添加剂等用途。
2、使用甘油菌时可不完全融解,在甘油菌表面蘸取少量涂布固体琼脂平板即可,也可完全融解后使用,但随着冻融次数的增加,菌株的活力会逐渐下降。