Anti-CD4 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,FC

• 抗体类型兔多抗

• 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250.

• 偶联Non-conjugated

• Anti-CD4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cell Membrane

• 数据链接P01730 Human
  P06332 Mouse
  P05540 Rat
  

• 其它名称CD 4 antibody
  CD4 (L3T4) antibody
  CD4 antibody
  CD4 antigen (p55) antibody
  CD4 antigen antibody
  CD4 molecule antibody
  CD4 receptor antibody
  CD4+ Lymphocyte deficiency, included antibody
  CD4_HUMAN antibody
  CD4mut antibody
  L3T4 antibody
  Leu3 antibody
  Ly-4 antibody
  Lymphocyte antigen CD4 antibody
  MGC165891 antibody
  OTTHUMP00000238897 antibody
  p55 antibody
  T cell antigen T4 antibody
  T cell antigen T4/LEU3 antibody
  T cell differentiation antigen L3T4 antibody
  T cell OKT4 deficiency, included antibody
  T cell surface antigen T4/Leu 3 antibody
  T cell surface antigen T4/Leu3 antibody
  T cell surface glycoprotein CD4 antibody
  T-cell surface antigen T4/Leu-3 antibody
  T-cell surface glycoprotein CD4 antibody
  W3/25 antibody
  W3/25 antigen antibody

  more

• Anti-CD4 antibody应用

  WB: 1:500
  ICC: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.

  

  Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.