Anti-SOAT2 antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human ACAT2 331-430/522

• 偶联Non-conjugated

• Anti-SOAT2 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Endoplasmic reticulum membrane; Multi-pass membrane protein.

• 其它名称Acat2
  acetoacetyl CoA thiolase
  acetoacetyl Coenzyme A thiolase
  Acetyl CoA acetyltransferase
  Acetyl CoA transferase like protein
  acetyl Coenzyme A acetyltransferase 2
  Acetyl-CoA acetyltransferase
  Acetyl-CoA transferase-like protein
  Acyl Coenzyme A cholesterol acyltransferase 2
  Cytosolic acetoacetyl-CoA thiolase
  cytosolic
  SOAT2
  SOAT2_HUMAN.

  more

• Anti-SOAT2 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  K562(Human) Cell Lysate at 30 ug
  Jurkat(Human) Cell Lysate at 30 ug
  Primary: Anti-SOAT2  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 40 kD
  Observed band size: 40 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SOAT2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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