Anti-MCU antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein within human MCU aa 50-250.

• 偶联Non-conjugated

• Anti-MCU antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Mitochondrion.

• 其它名称C109A_HUMAN antibody
  C10orf42 antibody
  Calcium uniporter protein mitochondrial antibody
  Ccdc109a antibody
  Coiled-coil domain-containing protein 109A antibody
  HsMCU antibody
  Mitochondrial Calcium Uniporter antibody

  more

• Anti-MCU antibody应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50 -1:200
  FC: 1:50-1:100

  Fig1: Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.
  Positive control:
  Lane 1: A549
  Lane 3: mouse spleen tissue
  Lane 2: HL-60
  Lane 4: mouse brain tissue
  

  

  Fig2: ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.

  

  Fig10: Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

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