Anti-ACADM antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,ICC

• 抗体类型兔多抗

• 免疫原Recombinant protein within human ACADM aa 10-250.

• 偶联Non-conjugated

• Anti-ACADM antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Mitochondrion matrix.

• 其它名称ACAD 1 antibody
  ACAD1 antibody
  Acadm antibody
  ACADM_HUMAN antibody
  Acyl coenzyme A dehydrogenase antibody
  Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
  FLJ18227 antibody
  FLJ93013 antibody
  FLJ99884 antibody
  MCAD antibody
  MCADH antibody
  Medium chain acyl CoA dehydrogenase antibody
  Medium chain fatty acyl CoA dehydrogenase antibody
  Medium chain specific acyl CoA dehydrogenase antibody
  Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
  Medium-chain specific acyl-CoA dehydrogenase antibody
  mitochondrial antibody

  more

• Anti-ACADM antibody应用

  WB:1:500-1:2,000
  ICC:1:50-1:200
  IHC-P:1:50-1:200
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of ACADM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Daudi cell lysate
  Lane 2: K562 cell lysate

  

  Fig2: ICC staining of ACADM in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibodyfor 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-ACADM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACADM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig5: Flow cytometric analysis of ACADM was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody(red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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