Anti-BOB1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein within Human BOB1 aa 10-180.

• 偶联Non-conjugated

• Anti-BOB1 antibody性能

• 形态Liquid

• 浓度1 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Nucleus.

• 其它名称B cell Oct binding protein 1 antibody
  B cell specific coactivator OBF 1 antibody
  B cell specific coactivator OBF1 antibody
  B-cell-specific coactivator OBF-1 antibody
  BOB 1 antibody
  BOB-1 antibody
  OBF 1 antibody
  OBF1 antibody
  OBF1_HUMAN antibody
  OCA B antibody
  OCA-B antibody
  OCAB antibody
  OCT binding factor 1 antibody
  OCT-binding factor 1 antibody
  POU class 2 associating factor 1 antibody
  POU domain class 2 associating factor 1 antibody
  POU domain class 2-associating factor 1 antibody
  Pou2af1 antibody

  more

• Anti-BOB1 antibody应用

  WB:1:500
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

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