Anti-DUSP4 antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human MKP-2 301-394/394

• 偶联Non-conjugated

• Anti-DUSP4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

• 其它名称Mitogen activated protein kinase phosphatase 2
  Dual specificity phosphatase 4
  Dual specificity protein phosphatase 4
  Dual specificity protein phosphatase hVH2
  DUSP 4
  HVH 2
  HVH2
  MAP kinase phosphatase 2
  Mitogen activated protein kinase phosphatase 2
  MKP 2
  MKP-2
  MKP2
  Serine/threonine specific protein phosphatase
  TYP
  VH 2
  VH1 homologous phosphatase 2
  VH2.

  more

• Anti-DUSP4 antibody应用

  WB:1:500-2000
  FC:1ug/Test

• 

  Fig1: Sample:
  RAW264.7(Mouse) Cell Lysate at 30 ug
  Primary: Anti-DUSP4 at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 44 kD
  Observed band size: 44 kD
  

  

  Fig2: Blank control:A431.
  Primary Antibody (green line): Rabbit Anti-DUSP4 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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