Anti-Dux antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within mouse Dux aa 200-500.

• 偶联Non-conjugated

• Anti-Dux antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Nucleus.

  
  

• 其它名称Double homeobox 4 antibody
  Double homeobox protein 10 antibody
  Double homeobox protein 4 antibody
  Double homeobox protein 4/10 antibody
  DUX10 antibody
  DUX4L antibody
  DUX4L1 antibody

  more

• Anti-Dux antibody应用

  WB:1:500-1:2,000
  IHC-P:1:50-1:200
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Hela cell lysate
  Lane 2: Jurkat cell lysate

  

  Fig2: Western blot analysis of Dux on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig5: Flow cytometric analysis of Dux was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody ((red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！