Anti-Afadin/I Afadin antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human AF6 341-440/1816

• 偶联Non-conjugated

• Anti-Afadin/I Afadin antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell junction, adherens junction. Note=Not found at cell-matrix AJs.

  
  

• 其它名称AF 6
  AF6
  AFAD_HUMAN
  Afadin
  ALL1-fused gene from chromosome 6 protein
  FLJ34371
  MLLT 4
  MLLT4
  Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4
  Myeloid/lymphoid or mixed lineage leukemia translocated to 4
  Protein AF 6
  Protein Af-6
  Protein AF6
  RP3 431P23.3
  5033403D15Rik
  Gm314
  I-afadin
  Mllt4
  S-afadin.

  more

• Anti-Afadin/I Afadin antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Hippocampus (Mouse) Lysate at 40 ug
  Primary: Anti-Afadin  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 136 kD
  Observed band size: 135/138 kD
  

  

  Fig2: Sample:
  Skin (Rat) Lysate at 40 ug
  Primary: Anti-Afadint 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 136 kD
  Observed band size: 135/138 kD
  

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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