Anti-CCDC94 antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CCDC94 15-120/323

• 偶联Non-conjugated

• Anti-CCDC94 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 其它名称Coiled coil domain containing protein 94
  FLJ10374
  MGC156684
  CCD94_HUMAN.

• Anti-CCDC94 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Testis (Mouse) Lysate at 40 ug
  Primary: Anti- CCDC94  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 37 kD
  Observed band size: 35 kD
  

  

  Fig2: Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CCDC94 Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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