Anti-CIDEC antibody

• 种属反应性Human,Mouse,Rat,Pig

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CIDEC 101-200/238

• 偶联Non-conjugated

• Anti-CIDEC antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus (By similarity). Endoplasmicreticulum (By similarity). Lipid droplet. Note=Diffuses quickly onlipid droplet surface, but becomes trapped and clustered at lipiddroplet contact sites, thereby enabling its rapid enrichment atlipid droplet contact sit

• 其它名称Cell Death Activator
  Cell death activator CIDE-3
  Cell Death Inducing DFFA Like Effector C
  Cell death inducing DFFA like effector protein C
  Cell death-inducing DFFA-like effector protein C
  CIDE 3
  CIDE3
  CIDE C
  CIDEC_HUMAN
  Fat specific protein 27
  Fat-specific protein FSP27 homolog
  FLJ20871
  FSP27.

  more

• Anti-CIDEC antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Epididymis (Mouse) Lysate at 40 ug
  Primary: Anti-CIDECat 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 27 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig3: Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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