Anti-ISX/Intestine specific homeobox antibody

• 种属反应性Human,Mouse,Rat,Cow,Horse,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox 81-180/245

• 偶联Non-conjugated

• Anti-ISX/Intestine specific homeobox antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

• 其它名称DKFZp781N2395
  Intestine-specific homeobox
  Isx
  ISX_HUMAN
  MGC138417
  OTTHUMP00000028558
  Pancreas intestine homeodomain transcription factor
  Pix 1
  RAX like homeobox
  RAX-like homeobox
  RAXLX.

  more

• Anti-ISX/Intestine specific homeobox antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

  

  Fig2: Sample:
  small intestine (Mouse) Lysate at 40 ug
  large intestine (Mouse) Lysate at 40 ug
  Primary: Anti-ISX/Intestine specific homeobox  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 27 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！