Anti-PER1 protein antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PER1 protein 751-850/1290

• 偶联Non-conjugated

• Anti-PER1 protein antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1

• 其它名称Circadian clock protein PERIOD 1
  Circadian clock protein PERIOD1
  Circadian pacemaker protein Rigui
  hPER 1
  hPER
  hPER1
  KIAA0482
  MGC88021
  PER 1
  PER
  PER1
  PER1 protein
  PER1_HUMAN
  Period 1
  Period circadian protein homolog 1
  Period drosophila homolog of
  Period homolog 1
  Period1
  RIGUI.

  more

• Anti-PER1 protein antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Muscle (Mouse) Lysate at 40 ug
  Primary: Anti-PER1 protein at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 136 kD
  Observed band size: 136 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PER1 protein) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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