Anti-Presenilin 1 + 2 antibody

• 种属反应性Human,Mouse,Rat,Chicken

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Presenilin-1 NTF subunit 321-430/467

• 偶联Non-conjugated

• Anti-Presenilin 1 + 2 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface. Note=Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1

  
  

• 其它名称AD 3
  AD4
  AD5
  Alzheimer disease 3
  Alzheimer disease 4
  FAD
  Presenilin 1
  Presenilin 2
  Presenilin-1
  Presenilin-2
  Presenilin1
  Presenilin1
  PS1
  PS2
  PS-1
  PS-2
  PSEN1
  PSEN2
  STM2
  PSN1_HUMAN
  PSN2_HUMAN.

  more

• Anti-Presenilin 1 + 2 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-Presenilin 1 + 2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 19/52 kD
  Observed band size: 50/52 kD
  

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Presenilin 1+2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Presenilin 1+2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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