产品详情
Anti-Dux antibody
种属反应性Mouse
验证应用WB,ICC
抗体类型兔多抗
免疫原Synthetic peptide within mouse Dux aa 520-570.
偶联Non-conjugated
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Nucleus.
其它名称
Double homeobox 4 antibody
Double homeobox protein 10 antibody
Double homeobox protein 4 antibody
WB: 1:500
ICC: 1:50-1:200
Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Non-transfected 293T cells lysate
Lane 2: His-tagged Dux transfected 293T cells lysate
Fig2: ICC staining of Dux in Non-transfected 293T and His-tagged Dux transfected 293T cells cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
问商家