Anti-Beta-galactosidase antibody

• 种属反应性Human

• 验证应用WB,IHC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human Beta-galactosidase aa 610-677.

• Anti-Beta-galactosidase antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Cytoplasm, Lysosome.

• 其它名称

  more

• Acid beta galactosidase antibody

• Acid beta-galactosidase antibody

• Beta galactosidase 1 antibody

• Anti-Beta-galactosidase antibody应用

  WB: 1:1,000-1:2,000
  IHC: 1:100-1:500

• 

  Fig1: Western blot analysis of Beta-galactosidase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A549 cell lysate
  Lane 2: Hela cell lysate

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！