Anti-Bmi1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein within human Bmi1 full sequence.

• 偶联Non-conjugated

• Anti-Bmi1 antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG2a

• 纯化方式Protein A purified.

• 亚细胞定位Nucleus, Cytoplasm

• 其它名称

  more

• B lymphoma Mo MLV insertion region (mouse) antibody

• B lymphoma Mo MLV insertion region 1 homolog antibody

• Bmi 1 antibody

• Anti-Bmi1 antibody应用

  WB: 1:1,000
  IHC-P: 1:200
  ICC: 1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: 293T
  Lane 2: Jurkat
  Lane 3: Hela
  Lane 4: MCF-7
  Lane 5: HepG2
  Lane 6: NIH/3T3
  Lane 7: PC12
  Lane 8: Mouse kidney
  Lane 9: Human kidney
  Lane 10: K562
  Lane 11: Human brain

  

  Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.

  

  Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

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