Anti-Bmi1 antibody

• 种属反应性Human,Mouse

• 验证应用WB,IHC-P,ICC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• Anti-Bmi1 antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG2a

• 纯化方式Protein affinity purified

• 亚细胞定位Cytoplasm. Nucleus.

• 其它名称

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• B lymphoma Mo MLV insertion region (mouse) antibody

• B lymphoma Mo MLV insertion region 1 homolog antibody

• Bmi 1 antibody

• Anti-Bmi1 antibody应用

  WB: 1:500-1:1,000
  ICC: 1:200
  IHC-P: 1:200-1:400

• 

  Fig1: Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.

  

  Fig2: ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

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