Anti-CD68 antibody

• 种属反应性Human

• 验证应用WB,IHC-P,ICC,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• Anti-CD68 antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cell membrane and Endosome membrane. Lysosome membrane.

• 其它名称

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• CD 68 antibody

• CD68 antibody

• CD68 antigen antibody

• Anti-CD68 antibody应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:1,000
  IHC-P: 1:200-1:1,000
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.

  

  Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.

  

  Fig3: Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution.
  Positive control: Line1: U937 Line1: Hela Line2: HepG2 Line3: Jurkat

  

  Fig4: ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.

  

  Fig7: Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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