Anti-HLA-B antibody

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• Anti-HLA-B antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Membrane

• 其它名称

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• AS antibody

• HLA B protein antibody

• HLA class I histocompatibility antigen, B alpha chain antibody

• Anti-HLA-B antibody应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

• 

  Fig1: Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.

  

  Fig2: Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.

  

  Fig3: Western blot analysis of HLA-B on Ramos(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.

  

  Fig4: ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.

  

  Fig7: Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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