Anti-Ribonuclease 3 antibody

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein within human Ribonuclease 3 aa 28-120.

• 偶联Non-conjugated

• Anti-Ribonuclease 3 antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein G purified.

• 亚细胞定位Secreted.

• 其它名称

  more

• Cytotoxic ribonuclease antibody

• ECP antibody

• ECP_HUMAN antibody

• Anti-Ribonuclease 3 antibody应用

  WB:1:500-1:2000
  ICC:1:100
  IHC-P:1:50-1:200
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of Ribonuclease 3 on U937 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  
  Lane 1: Anti-Ribonuclease 3 Antibody, (1:500).
  Lane 2: Anti-Ribonuclease 3 Antibody, (1:500), preincubated with the immunization protein.

  

  Fig2: ICC staining Ribonuclease 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ribonuclease 3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining Ribonuclease 3 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ribonuclease 3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ribonuclease 3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ribonuclease 3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig6: Flow cytometric analysis of Ribonuclease 3 was done on Hela cells. The cells were fixed, permeabilized and stained with Ribonuclease 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！