Anti-Transferrin antibody

• 种属反应性Human

• 验证应用IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Native protein.

• 偶联Non-conjugated

• Anti-Transferrin antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein G purified.

• 亚细胞定位Secreted.

• 其它名称

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• Apotransferrin antibody

• Beta 1 metal binding globulin antibody

• Beta-1 metal-binding globulin antibody

• Anti-Transferrin antibody应用

  IHC-P:1:100
  FC:1:50-1:100

• 

  Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodat 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig3: Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with Transferrin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.

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