别称

NIFUN

 宿主

Rabbit

 适应种属

Human, Mouse, Rat

 亚型

IgG

 验证应用

ELISA, WB, IHC, IF, IP

产品信息

产品名称

anti- ISCU antibody

货号

QSab04402

规格

100μg

状态

liquid

纯化方法

Immunogen affinity purified

纯度

≥95% as determined by SDS-PAGE

宿主

Rabbit

克隆性

polyclonal

亚型

IgG

存储

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

背景介绍

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020) . First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity) .

免疫原信息

免疫原

iron-sulfur cluster scaffold homolog (E. coli)

别称

NIFUN

分子量

14-15 kDa, 18-19 kDa

应用

验证应用

ELISA, WB, IHC, IF, IP

建议稀释比例

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200