甲型流感病毒 百欧博伟生物 ATCC菌种

菌种简介

平台编号：Bio-50976

规格：Frozen

拉丁属名：Influenza A Virus (H3N2)

菌株名称：甲型流感病毒

用途：研究

注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用

详细信息

ATCC? Number:VR-1679?

Classification: Orthomyxoviridae, Influenzavirus A

Agent: Influenza A virus (H3N2)

Strain: A/Hong Kong/8/68 (TC-adapted)

Original Source: Isolated from a patient in Hong Kong, 1968

Depositors: M. T. Coleman and ATCC

Biosafety Level:2

Shipped: frozen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Host Organism : Production Host : MDCK (ATCCCCL-34) Alternate Hosts:CE, MDCK, MkK(monkey kidney cells)

Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1679 should be grown in serum free media that contains 1ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).

Effect : CPE (cell rounding, degeneration, sloughing)

Store at : -70℃ or colder

Comments : Derived by adaptation of egg-passage A/Hong Kong/8/68 (ATCCVR-544) to MDCK (ATCC-34) cells. (Note: ATCCVR-544 and ATCCVR-1679 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture.)

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