TFPI2 Protein, Human, Recombinant (His Tag)

别称：PP5 Protein, Human; REF1 Protein, Human; TFPI-2 Protein, Human

纯度:> 95 % as determined by SDS-PAGE

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method

生物活性：Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.

蛋白构建：A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.

NP No.：NP_006519.1

表达宿主：HEK293 Cells

种属：Human

预测 N 端：Asp 23

分子量：The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.

缓冲液：Lyophilized from sterile PBS, pH 7.4.

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

运输方式：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶：A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。