产品详情
复制子: ColE1 Ori
质粒分类: 枯草杆菌质粒;枯草整合质粒;枯草分泌质粒
质粒大小: 8965bp
原核抗性: Amp
克隆菌株: DH5a
培养条件: 37度
质粒属性
载体宿主: 枯草杆菌
载体用途: 蛋白表达
基因种属:
基因类型: ORF
原核抗性: Amp
真核抗性:
荧光蛋白:
质粒简介
pIEFBPR质粒是一个枯草整合型分泌载体,该质粒的参考文献:mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.