产品详情
HAT培养基添加剂 (50×)
HAT Supplement (50×)
产品规格:10ml
保存条件: Store at -20℃.
产品介绍:
HAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells.
γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma;
Application:
HAT Media Supplement (50×) Hybri-Max has been used:
in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones.
in the generation of mAbs (monoclonal antibodies).
in the luciferase-based bioassay (LUC) assay.
Reconstitution:
Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!