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上海博尔森生物科技有限公司
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溶菌酶 (≥40KU/mg, 进口)

Lysozyme from chicken egg white

产品规格: 1g

CAS: 12650-88-3

保存条件: -20℃保存

产品介绍:

CAS号:12650-88-3

分子量:single-chain 14.3kDa

MDL:MFCD00131557

级别/活性:BR,≥40,000 units/mg protein

纯度:≥90% (HPLC)

外观/性状:lyophilized powder

酶活性定义:One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

chem/physiol Actions:

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

L6876 (Sigma-Aldrich)

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

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