产品详情
平台编号:Bio-132792
产品信息
名称 OE19 (人食管癌细胞) (STR鉴定正确)
别称 OE-19; JROECL 19; JROECL19; OEC19
种属 人
生长特性 贴壁细胞
细胞形态 上皮细胞样
生长培养基 RPMI-1640+10% FBS+1% P/S
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO温度:液氮
培养条件 气相:空气,95%;CO2,5%温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3天/次
参考资料(来源文献)
背景描述 OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2 . All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
年龄(性别) 男,72岁
组织来源 食管癌
细胞类型 肿瘤细胞
肿瘤类型 食管癌细胞
生物安全等级 1
倍增时间 ~50-60 hours (DSMZ=ACC-700)
致瘤性 裸小鼠致癌
保藏机构 ECACC; 96071721
STR鉴定
STR位点信息
Amelogenin X
CSF1PO 11,13
D2S1338 18,19
D3S1358 15,18
D5S818 11,14
D7S820 8
D8S1179 13,15
D13S317 9,11
D16S539 12,13
D18S51 12
D19S433 13
D21S11 30
FGA 23,26
PentaD 9
PentaE 5,8
TH01 8,9
TPOX 8
vWA 16,17,18
D6S1043 19
D12S391 20
D2S441 14,15
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