The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. Recombinant Human TGF-β1 derived from HEK293 cells is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.

Source: HEK293 cells

Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor

AA Sequence (monomer): ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.
Recombinant Human TGF-β1 (HEK293 derived) Biological Activity Graph Recombinant Human TGF-β1 (HEK293 derived) WHO Comparison Graph

Calculated Molecular Weight: 25 kDa

Accession Number: P01137

Gene ID: 7040

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Cross Reactivity Cited in References:
Chicken, Cow, Dog, Donkey, Frog, Human, Human + Mouse, Mink, Monkey, Mouse, Mouse + Virus, Pig, Rabbit, Rat