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Rotavirus A

VR-3392 ™

This product is an ATCC manufactured and accessioned progeny of VR-2551 cited in US Pat. No. 5,626,851.

Product category

Viruses

Product type

Animal virus

Classification

Reoviridae, Rotavirus, Rotavirus A

Strain designation

WI61

Genome sequenced strain

Yes

Isolation source

Human infant

Geographical isolation

United States; Pennsylvania; Philadelphia

Applications

Infectious disease research

Enteric disease research

Product format

Frozen

Storage conditions

-70°C or colder

General

Specific applications

Enteric research

Characteristics

Comments

May cause gastroenteritis in infants

Propagate in cells fed with serum-free medium containing 0.5 µg/mL purified trypsin

Handling information

Host

MA-104 Clone 1 (ATCC CRL-2378.1)

Effects

cell rounding; cell sloughing; CPE; plaque formation

Complete medium

DMEM (ATCC 30-2002) + 8 µg/mL Gibco Trypsin (Invitrogen 27250-018)

Temperature

36°C

Atmosphere

95% Air, 5% CO2

Incubation

1 to 4 days at 36°C, a 5% CO2 in air atmosphere is recommended

Recommendation for infection

For best results, cells should be 24 to 48 hours old and 100% confluent.

Handling notes

Cultivate in cell lines (MA-104, CV-1) fed with serum-free medium supplemented with 8 µg/mL trypsin.

Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.

Key abbreviations

bp, Base pairs

CO2, Carbon dioxide

CPE, Cytopathic effect

CV-1, African green monkey kidney cells

DMEM, Dulbecco's Modified Eagle's Medium

ELISA, Enzyme-linked immunosorbent assay

NCBI, National Center for Biotechnology Information

MA-104, Embryonic rhesus monkey kidney cells

µg, Microgram

mL, Milliliter

PCR, Polymerase chain reaction

RT-PCR, reverse-transcriptase polymerase chain reaction

TC, Tissue culture

TCID[50], Median tissue culture infective dose

Quality control specifications

Verification method

Whole-genome Sequencing

History

Deposited as

ATCC accessioned progeny of Rotavirus A strain WI61 cited in US Pat. No. 5,626,851 as VR-2551.

Type of isolate

Human

Patient age

infant

Special collection

NCRR Contract

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.