平台编号：Bio-137155

Aspergillus nidulans (Eidam) Winter

MYA-3632 ™

An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.

Product category

Fungi

Strain designation

AN1

Type strain

No

Isolation source

Human biopsy (lung)

Geographical isolation

United States; Maryland; Bethesda

Applications

Agricultural research

Product format

Frozen

Storage conditions

-80°C or colder

General

Specific applications

Biomedical Research and Development Material

Preceptrol

No

Handling information

Medium

ATCC Medium 325: Malt extract agar (Blakeslee's formula)

ATCC Medium 336: Potato dextrose agar (PDA)

ATCC Medium 324: Malt extract agar

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.

Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

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