平台编号：Bio-137149

Penicillium cremeogriseum Chalabuda

18323 ™

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Classification

Fungi, Dikarya, Ascomycota, Pezizomycotina, Eurotiomycetes, Eurotiomycetidae, Eurotiales, Aspergillaceae, Penicillium

Strain designation

VKM F-1034 [ATCC 18320, CBS 223.66, FRR 1734, IMI 197492, NRRL 3389]

Type strain

Yes

Isolation source

Forest soil

Geographical isolation

USSR

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Preceptrol

No

Characteristics

Morphology

After 5 days on Blakeslee’s MEA at 25°C, colonies are grey or greyish-green, low, velutinous or cottony, reverse pale yellow. Conidia subspherical or ellipsoidal, smooth-walled.

Handling information

Medium

ATCC Medium 200: YM agar or YM broth

ATCC Medium 325: Malt extract agar (Blakeslee's formula)

ATCC Medium 336: Potato dextrose agar (PDA)

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water.

Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

Incubate the inoculum at the propagation conditions recommended.

Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain. 

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications