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 Megasphaera cerevisiae Engelmann and Weiss

43236 ™

Product category

Bacteria

Product type

Extremophile

Strain designation

DSM 20461 [Sp III 2e]

Type strain

No

Isolation source

Spoiled beer

Geographical isolation

Germany

Product format

Frozen

Storage conditions

-80°C or colder

General

Specific applications

Characterization

Preceptrol

No

Handling information

Medium

ATCC Medium 1528: PY-fructose (ATCC medium 1524) with 10 g fructose per liter of medium

Temperature

26°C

Atmosphere

Anaerobic

Handling procedure

Open vial according to enclosed instructions.

Under anaerobic conditions, withdraw 0.5 mL of the recommended broth from a single test tube (5 to 6 mL) and rehydrate the vial contents.

Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 mL each from the suspension.  Streak several blood plates to check for colonial morphology and purity.

Incubate tubes under an anaerobic atmosphere at 26°C.  Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check. 

In 24 to 48 hours, growth should be evident by turbidity that settles to the bottom of the broth.  No growth should occur on agar plate incubated aerobically.

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber, or

Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber,

Loose screw caps on test tubes in an activated anaerobic gas pack jar, or

Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

Handling notes

Colonies on anaerobic blood plates are pinpoint, punctiform, colorless, and transparent.

Allow the culture to grow in the primary broth tube before transferring it to other tubes.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as

Megasphaera cerevisiae Engelmann and Weiss

Depositors

DSM

Chain of custody

ATCC <-- DSM <-- H Seidel Sp III 2e

Type of isolate

Food & Beverage

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