平台编号：Bio-136940

Synechococcus sp.

27264 ™

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Cyanobacterium known to produce the restriction endonuclease AquI.

Product category

Bacteria

Strain designation

PCC 7002 [PR-6]

Type strain

No

Genome sequenced strain

Yes

Applications

Bioinformatics

Product format

Frozen

Storage conditions

-80°C or colder

General

Specific applications

Produces restriction endonuclease AquI

Biotechnology

Preceptrol

No

Characteristics

Comments

Taxonomy

Genome sequenced strain

Handling information

Medium

ATCC Medium 1047: MN Marine medium (ATCC Medium 957) with 20 mcg/L of vitamin B12

Temperature

26°C

Atmosphere

Aerobic

Incubation

Under light intensity of 2,000-3,000 lux

Handling procedure

Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.

Aseptically transfer the entire contents to a 5-6 mL tube of #1047 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.

Use several drops of the primary broth tube to inoculate a #1047 agar slant.

Incubate at 26°C for 7-14 days under 2000-3000 lux light.  It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth and to enhance exposure to light.

Handling notes

Good growth, indicated by green, plant-like growth in broth or on the slant, should occur after one to two weeks of incubation. Examine cells microscopically to assure that they are intact and healthy. At this time additional test tubes or flasks can be inoculated. A 5% inoculum is recommended (i.e. 5 mL of culture to 100 mL fresh medium).

To minimize change in a culture, it is recommended that a frozen seed stock be established from early passage cells. This may be accomplished by propagating the strain under ideal conditions, utilizing recommended medium, temperature and light. Prepare a concentrated cell suspension, after good growth is achieved. If grown in broth, pellet the cells by centrifugation. Decant the supernatant and resuspend the pellet in fresh #1047 broth using 1/10 or less of the original volume. For slant cultures, wash cells off the agar surface with a minimal amount of #1047 broth so that a concentrated cell suspension is attained. Add 50% DMSO solution to the concentrated cell suspension so that the final concentration of DMSO in the suspension is 5%. Dispense small aliquots (0.5 to 1 mL) of the suspension into small sterile vials. Store the vials at -50°C or below.

When needed, remove vials from storage, thaw contents in a 37°C water bath and inoculate into recommended medium. A minimum of 0.2 mL of the thawed stock should be used to inoculate 5 mL of broth or 1 agar slant.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method

Whole-genome Sequencing

History

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