平台编号：Bio-135423

Human herpesvirus 5

VR-3348™

Human herpesvirus 5 strain UL32-EGFP-HCMV-TB40 is propagated in MRC-5 cells (ATCC CCL-171). This strain was sourced from the in vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination; this is useful for following intracellular traffic of virus particles.

Product category

Viruses

Product type

Animal virus

Classification

Herpesviridae, Cytomegalovirus, Human betaherpesvirus 5

Strain designation

UL32-EGFP-HCMV-TB40

Common name

Cytomegalovirus

Isolation source

In vitro recombination of HCMV strain TB40 with a plasmid carrying the TB40 UL32 gene fused to GFP.

Applications

Infectious disease research

Product format

Frozen

Storage conditions

-70°C or colder

General

Specific applications

Tracking intracellular traffic of virus particles.

Characteristics

Comments

Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination.  Useful for following intracellular traffic of virus particles.

Handling information

Host

MRC-5 (ATCC CCL-171)

Effects

CPE; cell rounding; cell enlargement

Complete medium

EMEM (ATCC 30-2003) supplemented with 2% FBS (ATCC 30-2020)

Temperature

37°C

Incubation

7-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Recommendation for infection

Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (eg, 1 mL per 25 cm2) diluted to provide an optimal MOI (eg, 0.1 to 1.0). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Handling notes

Recombinant HCMV virus with GFP fused to the C terminus of the tegument phosphoprotein pp150, the product of the UL32 gene. Because GFP is associated with a structural protein, virus particles fluoresce green under appropriate illumination.  Useful for following intracellular traffic of virus particles.

Key abbreviations

°C, Degrees Celsius

CO2, Carbon dioxide

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

MOI, Multiplicity of infection

Quality control specifications

History

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