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Sporothrix schenckii Hektoen et Perkins

58251 ™

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Strain designation

de Perez 2211183

Type strain

No

Genome sequenced strain

Yes

Isolation source

Clinical isolate

Geographical isolation

United States; Puerto Rico

Applications

Agricultural research

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

Biomedical Research and Development Material

Preceptrol

No

Characteristics

Comments

Yeast-mycelium transition

Genome sequencing strain (Broad Institute, USA)

Handling information

Medium

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 200: YM agar or YM broth

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

For freeze-dry (lyophilized) ampoules:

Open an ampoule according to enclosed instructions.

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.

Aseptically transfer the suspension back into the test tube of sterile distilled water.

Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.

Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.

Incubate the inoculum at the propagation conditions recommended.

Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain. 

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

History

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